msmR [2019-05-30 19:17:28]

transcriptional regulator of the [gene|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR]-[gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]-[gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]-[gene|4E766839240C8F7D56D87DF9E7B99350A83B5FF7|melC]-[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA] operon

locus: BSU30260

pI: 6.45

mw: 38.24 kDa

protein length: 344 aa

gene length: 1035 bp

function: regulation of melibiose utilization

product: transcriptional regulator ([SW|LacI family])

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.21|Utilization of melibiose]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 3,096,782 3,097,816

The protein

Catalyzed reaction/ biological activity

• represses the the [gene|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR]-[gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]-[gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]-[gene|4E766839240C8F7D56D87DF9E7B99350A83B5FF7|melC]-[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA] operon in the absence of melibiose or raffinose [pubmed|31138628]

Protein family

• [SW|LacI family]

Structure

• [PDB|1VPW] (E. coli [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR], 29% identity) [pubmed|9628480]

Expression and Regulation

Operons

• genes: [gene|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR]-[gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]-[gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]-[gene|4E766839240C8F7D56D87DF9E7B99350A83B5FF7|melC]-[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22900538,31138628], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|22900538], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|MelR]: repression, [pubmed|31138628], in [regulon|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|MelR regulon]

  regulation

  • induced by melibiose or raffinose ([protein|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|MelR]) [pubmed|31138628]
  • repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|22900538,31138628]
  • there is an additional RNA "upshift" signal in front of the [gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE] gene suggestive of a [gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE]-[gene|05BFEA711D82395A55B505E5A38286BE2F570350|melD]-[gene|4E766839240C8F7D56D87DF9E7B99350A83B5FF7|melC]-[gene|CDE9EF9CFB799794D525F12DEC8C4190C6298575|melA] mRNA [Pubmed|22383849]. However, there is no promoter in front of [gene|C3D7C64AD0E58BF326257E23136466A237138B50|melE], suggesting that this mRNA may be the product of mRNA processing [pubmed|31138628]

Biological materials

Mutant

• MGNA-A802 (msmR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/802 NBRP B. subtilis, Japan]
• BKE30260 ([gene|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCCCTCTCATCT, downstream forward: _UP4_TAAGCAAAGATTCATCACGA
• BKK30260 ([gene|58276E6FEF9388BBC7FB79BB5A3A1EF3C3DA5BD5|melR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCCCTCTCATCT, downstream forward: _UP4_TAAGCAAAGATTCATCACGA

References

• 22383849,22900538,9628480,31138628